Figure 3. Transfection with targeting siRNAs suppressed TβRII protein expression by immunofluorescence staining. Untreated SRA01/04 cells (A-C), SRA01/04 cells treated with 80 nM scrambled siRNA for 48 h (D-F), or treated with 80 nM hT3 (G-I) were observed under immunofluorescence microscope. Staining of nuclei with Hoechst 33342 (blue) is shown in A, D, and G. TβRII expression is shown in B, E, and H. Compared with the control cells (B and E), TβRII staining of cells treated with hT3 siRNA was weaker (H). The morphology after the siRNA treatment were somewhat altered. Note the exclusively cytoplasmatic localization of TβRII expression (C, F, and I). Bar: 25 μm.