Figure 1. Qualitative analysis of optimal transfection of different concentration of Cy3-labeled scrambled siRNA for SRA01/04 cells. Distribution of Cy3-labeled scrambled siRNA (red fluorescence) in SRA01/04 cells at 6 h after scrambled siRNA transfection with a final concentration gradient of 20 (A), 40 (B), 50 (C), 60 (D), or 80 nM (E). Cells incubated with Lipofectamine™ 2000 reagent alone (no siRNA) served as blank control which did not show fluorescence appearance (F). Bar: 25 μm.