Figure 7. Gene expression in corneas
as detected by reverse transcription and real-time quantitative
polymerase chain reaction. The relative expression levels of
each gene in the experimental groups over naïve mice were
obtained by the ΔΔCt method, as detailed in the Methods section.
Since the ΔCt for naïve mice was obtained by comparing Ct with
that of reference gene ribosomal protein L5 (RPL5), the
expression level for this group also has a standard deviation
over 1.0. * p<0.05 (n=3) as compared between the two groups.
Note (#): Since matrix metalloproteinase (MMP)-13 was not
detectable in the naïve mice, with the result that the ΔΔCt
method did not work, the relative levels of this gene compared
with RPL5 in the experimental groups was obtained by the ΔCt