Figure 7. Expression and localization of CTRP5 in the pig eye. A: Expression of pCTRP5 was studied by qRT–PCR using total mRNA extracted from different tissues of a 222-day-old pig eye. The pCTRP5 expression is presented as bars using an arbitrary scale on the y axis. Values are presented as mean (±SEM) of three independent observations after normalization with the control gene (HGPRT) B: western blot analysis of CTRP5 protein extracted from a 222 days pig. Retina (lane 1), optic nerve (lane 2), lens (lane 3), RPE (lane 4), ciliary body (lane 5), Choroid (lane 6). Detection with an anti-CTRP5 antibody shows significant expression of pCTRP5 protein in the RPE with an expected size molecular weight of approximately 31 kDa. C: Localization of CTRP5 in retinal sections as evaluated by IHC analysis of retinal sections with human monoclonal anti-CTRP5 antibody and Alexa Fluor 488 staining (green, arrows), nuclei (*) stained with DAPI (blue). D: Localization of CTRP5 in the ciliary body as shown by IHC with human monoclonal anti-CTRP5 antibody and Alexa Fluor 555 (red, arrows), nuclei stained with DAPI (blue).