Figure 5. Western blot analysis of Cos-7 cells lysates transfected with pig CTRP5 promoter constructs. A: Cos-7 cells were transfected with pEF-CTRP5 wt ST (lane 1), pEF-CTRP5 mt ST (lane 2), pCTRP5 wt T (lane 3), and pCTRP5 mt T (lane 4) and the cell lysates were incubated with anti-V5 antibody. B: The membrane was stripped and incubated with anti-CTRP5 polyclonal antibody. A single protein with as size of approximately 31 kDa was identified using either antibodies indicating the V5 tagged CTRP5 protein is being expressed (A and B). The putative pCTRP5 promoter is capable of expressing the V5 tagged protein confirming the functionality of the pCTRP5 promoter (A lanes 3 and 4; B lanes 3 and 4).