Figure 3 of Kandpal, Mol Vis 2012; 18:1123-1146.

Figure 3. Cyanine (SYBR) green real-time quantitative polymerase chain reaction (qPCR) validation was performed for selected transcripts. The transcripts were amplified as described in Methods section. Three technical replicates were performed for three biologic replicate samples of RNA isolated from nondiabetic animals, diabetic animals and diabetic animals treated with either inhibitor of receptor for advanced glycation endproducts (RAGE) indicated as RI, or an inhibitor of p38 mitogen activated protein kinase (MAPK) designated as p38. The bars (left to right) represent samples corresponding to diabetic animals, diabetic animals treated with RAGE inhibitor, and diabetic animals treated with p38 MAPK inhibitor, respectively. The error bars represent standard deviation from the mean value (n=9). The levels of transcript in diabetic and treated animals were determined relative to the levels present in nondiabetic controls.