Figure 4. Accumulation of cationic and neutral liposomes was observed in flatmount preparations. After the scanning laser ophthalmoscope (SLO) images described in Figure 3 were taken, choroidal-scleral flatmounts were prepared from the mice treated with cationic liposomes (CL)-Oregon green (OG) or neutral liposomes labeled with OG. Images were taken using fluorescence microscopy. Representative ones are shown in A. They were evaluated the same way as the SLO images (B). Values for CL-OG at d10 and d14 were significantly higher compared to those of neutral liposomes. The results confirmed that the kinetics were the same as for the SLO images. Data are means obtained from five mice. Error bars indicate SEM, and asterisks indicate statistical significance (p<0.05 as compared to d1).