Figure 2. Kinetics of accumulation of cationic liposomes (CL)- indocyanine green (ICG) or CL-Oregon green (OG) was observed with the SLO in vivo. A: ICG-CL were injected at d14, and scanning laser ophthalmoscope (SLO) images were recorded in a single choroidal neovascularization (CNV). While images taken before injection of ICG (0 min) showed no signal, the ICG fluorescence (795 nm excitation, 830 nm emission) became detectable in the CNV after 10 min. Maximal intensity was observed between 30 min and 90 min with a slow decrease afterwards. Other parts of the fundus were not specifically stained. B: OG-CL were injected at d10, and SLO images were recorded in a single CNV. This series shows the early signal in the capillaries 2–3 min after injection that disappears quickly [8] while the OG-CL signal in the CNV is coming up later and for a longer time as shown in A. C: ICG-CL (40%) and CL-OG were mixed and injected at d14, and SLO images were recorded in a single CNV. Note that the OG signal is somewhat weaker. IR: CNV lesion in infrared modus.