Figure 4. Full-field electroretinogram responses and apoptosis detection in Rdh13^+/+ and Rdh13^−/− mice. A: The scotopic electroretinogram responses of Rdh13^+/+ and Rdh13^−/− mice at 10 months of age were recorded. B: The amplitudes of a- and b-waves for either genotype was plotted as the mean±SD (n=5, each group), *: p>0.05. C: The terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining showed that there was no obvious apoptosis in both Rdh13^+/+ and Rdh13^−/− mice at 10 months of age. D: Apoptosis genes expression in Rdh13^+/+, Rdh13^+/−, and Rdh13^−/− mice was detected by western blots. There was no increased expression level of apoptosis genes in all genotypes, which was in accordance with the result of TUNEL.TNF-α, tumor necrosis factor alpha; Fas, TNF receptor superfamily member 6; Bax, B-cell lymphoma 2-associated X protein; P65, nuclear factor-kappa B P65; RPE, retinal pigment epithelia; OS, outer segments; IS, inner segments; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer.