Figure 3. Localization, retinal histology, and thickness measurements for the Rdh13 knockout mice. A: Immunofluorescence localization of Rdh13 (green) was shown in 3-month-old Rdh13^+/+ and Rdh13^−/− retina paraffin sections. B: western blot analysis of RDH13 protein in wild-type (WT) and homozygous mice revealed that there was no expression of Rdh13 in the retinas of Rdh13 knockout mice. C: Semi-thin sections of WT and homozygous mice retinas revealed no major differences in retinal histology at 3 and 10 months of age. D: Transmission electron microscopy (TEM) of the photoreceptor outer and inner segments and the outer nuclear layer in Rdh13^+/+ and Rdh13^−/− mice at 10 months of age revealed no apparent abnormalities. E: The outer-plus-inner-segment and outer nuclear layer thickness for Rdh13^−/− and WT mice at the ages of 3 months and 10 months was valued. Values were mean±SD (n=5, each group). There were no statistically significant differences between the two genotypes at any distance point. ONH, optic nerve head; RPE, retinal pigment epithelia; OS, outer segments; IS, inner segments; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer.