Table 1 of
Abu-Amero, Mol Vis 2012; 18:1004-1009. Table 1 of
Abu-Amero, Mol Vis 2012; 18:1004-1009.
Table 1. Primer sequences, PCR annealing temperature and amplicon size for the myocilin gene.
Exon |
Primer sequence |
Annealing temp (°C) |
Amplicon size (bp) |
|---|---|---|---|
| Pro-F | TGTAAAACGACGGCCAGTGCTGGCTGTTATTTTTCTCTGT | 55 | 592 |
| Pro-R | CAGGAAACAGCTATGACCCAGAAGCAGCAGCTGGACA | ||
| 1F | TGTAAAACGACGGCCAGTCACCCATCCAGGCACCTC | 57 | 775 |
| 1R | CAGGAAACAGCTATGACCGAGCGCCTGTAGCAGGTCA | ||
| 2F | TGTAAAACGACGGCCAGTTGGCCGGCAGCCTATTTA | 57 | 325 |
| 2R | CAGGAAACAGCTATGACCTGGGTGGGCATTTACCCTAT | ||
| 3AF | TGTAAAACGACGGCCAGTCATCTACTGGCTCTGCCAAG | 57 | 850 |
| 3AR | CAGGAAACAGCTATGACCAAGTTGTCCCAGGCAAAGAG | ||
| 3BF | TGTAAAACGACGGCCAGTTGACTACAACCCCCTGGAGA | 57 | 700 |
| 3BR | CAGGAAACAGCTATGACCCTGCAATCACATCTCCCAAC |