Figure 4. The reactivity of the peptide 3R antibody toward T6 SA peptide isomers. Results of ELISA assay for determining the specificity of the anti-peptide 3R antibody using αA-crystallin T6 SA peptides (TVLDAGISEVR), in which the Ser residue following the Asp residue was replaced by Ala, and in which the 4 different Asp isomers (L-α-Asp-, L-β-Asp-, D-α-Asp and L-β-Asp) were included. ELISAs were performed with a combination of a 100 times dilution for the first antibody and a 10,000 times dilution for the second antibody. The reactivity of the peptides can be expressed as a percentage using the reactivity toward T18 Dβ as 100%. (n=3, ±2σ [sigma]).