Figure 2. The reactivity of the peptide 3R antibody toward T18 peptide isomers. Results of ELISA assay for determining the specificity of the anti-peptide 3R antibody using 4 different αA-cystallin T18 isomeric peptides (IQTGLDATHAER) containing 4 Asp isomers, L-α-Asp-, L-β-Asp-, D-α-Asp and L-β-Asp. ELISAs were performed with a combination of a 100 times dilution for the first antibody and a 10,000 times dilution for the second antibody. The reactivity of the isomeric peptides can be expressed as a percentage using the reactivity toward T18 Dβ as 100%. (n=3, ±2σ [sigma]).