Table 1 of
Chen, Mol Vis 2012; 18:989-995. Table 1 of
Chen, Mol Vis 2012; 18:989-995.
Table 1. Primers used for PCR and sequencing of PAX6.
Target |
Exon ID |
Primer sequence (5′-3′) |
Annealing temperature (°C) |
Product length (bp) |
|---|---|---|---|---|
| 1a* | E1aF | AGCTGTGCCCAACTCTAGCC | 57 | 399 |
| E1aR | TTCCATCTTTGTATGCCTCCTT | |||
| 1 | E1F | CGGAGCCGAAAACAAGTG | 57 | 388 |
| E1R | GAGTGTGGGTGAGGGAAGTG | |||
| 2 | E2F | CCACTTCCCTCACCCACAC | 60 | 422 |
| E2R | CTCCTGCGTGGAAACTTCTC | |||
| 3 | E3F | AAGTGGGATCCGAACTTGC | 57 | 349 |
| E3R | CAGCCACCACAGAACTTGC | |||
| 4 | E4F | CAAGCCCCAAAGGGTAGATT | 57 | 286 |
| E4R | CGAAGTCCCAGAAAGACCAG | |||
| 5 | E5F | GGCTGGTGGTCCTGTTGTCCTT | 58 | 441 |
| E5R | GAGGGCGTTGAGAGTGGAG | |||
| 6,7 | E6–7F | AAGCAAGGTCAGCACAAAAATAAATT | 64 | 648 |
| E6–7R | GGAGGAGGTAAAGAGGAGAGAGCATT | |||
| 8 | E8F | TAAGGTTGTGGGTGAGCTGAGATG | 66 | 315 |
| E8R | GGGAGAGTAGGGGACAGGCAAAGG | |||
| 9 | E9F | TTTGGTGAGGCTGTCGGGATATAAT | 58 | 415 |
| E9R | TGCCCAGAGAAATAAAAAGACAGAAA | |||
| 10 | E10F | TTGGTTGGAGGTAATGGGAGTGG | 61 | 334 |
| E10R | TGGCAGCAGAGCATTTAGCAGAC | |||
| 11,12 | E11–12F | GGGGCTGGGCTCGACGTAG | 62 | 438 |
| E11–12R | GCCACCACCAGCCGCACTTA | |||
| 13 | E13F | GGGGCTGTGGCTGTGTGATGT | 61 | 333 |
| E13R | CCCCAGGGACAAGGAAAGCAA | |||
| 14 | E14F | CCAAACATGCAAACAAACAGAGGA | 52 | 570 |
| E14R | TTCCAACTGATATCGTGCCTTCTG |