Table 1 of
Wang, Mol Vis 2012; 18:968-973. Table 1 of
Wang, Mol Vis 2012; 18:968-973.
Table 1. Primer sequences for PCR amplification
Gene |
Forward Primers (5′→3′) |
Reverse Primers (5′→3′) |
Annealing Temperature (°C) |
Product Size (bp) |
|---|---|---|---|---|
| CRYAA-1 | AGCAGCCTTCTTCATGAGC | CAAGACCAGAGTCCATCG | 62 | 584 |
| CRYAA-2 | GGCAGGTGACCGAAGCATC | GAAGGCATGGTGCAGGTG | 62 | 550 |
| CRYAA-3 | GCAGCTTCTCTGGCATGG | GGGAAGCAAAGGAAGACAGA | 62 | 511 |
| CRYAB-1 | AACCCCTGACATCACCATT | AAGGACTCTCCCGTCCTAG | 62 | 352 |
| CRYAB-2 | CCATCCCATTCCCTTACCT | GCCTCCAAAGCTGATAGCA | 60 | 237 |
| CRYAB-3 | TCTCTCTGCCTCTTTCCTC | CCTTGGAGCCCTCTAAATC | 60 | 477 |
| CRYBA1–1 | GGCAGAGGGAGAGCAGAGTG | CACTAGGCAGGAGAACTGGG | 60 | 550 |
| CRYBA1–2 | AGTGAGCAGCAGAGCCAGAA | GGTCAGTCACTGCCTTATGG | 60 | 508 |
| CRYBA1–3 | AAGCACAGAGTCAGACTGAAGT | CCCCTGTCTGAAGGGACCTG | 60 | 463 |
| CRYBA1–4 | GTACAGCTCTACTGGGATTG | ACTGATGATAAATAGCATGAACG | 60 | 355 |
| CRYBA1–5 | GAATGATAGCCATAGCACTAG | TACCGATACGTATGAAATCTGA | 60 | 597 |
| CRYBA1–6 | CATCTCATACCATTGTGTTGAG | CATCTCATACCATTGTGTTGAG | 62 | 528 |
| CRYBB1-1 | CCCTGGCTGGGGTTGTTGA | TGCCTATCTGCCTGTCTGTTTCTC | 58 | 620 |
| CRYBB1-2 | TAGCGGGGTAATGGAGGGTG | AGGATAAGAGTCTGGGGAGGTGG | 58 | 664 |
| CRYBB1-3 | CCTGCACTGCTGGCTTTTATTTA | TCTCCAGAGCCCAGAACCATG | 60 | 475 |
| CRYBB1-4 | CCAACTCCAAGGAAACAGGCATA | CCTCCCTACCCACCATCATCTC | 60 | 491 |
| CRYBB1-5 | TAGACAGCAGTGGTCCCTGGAGA | AGCACTGGGAGACTGTGGAAGG | 60 | 416 |
| CRYBB1-6 | CCTAGAAAAGGAAACCGAGGCC | AGCGAGGAAGTCACATCCCAGTA | 60 | 551 |
| CRYBB2–1 | GTTTGGGGCCAGAGGGGAGTGGT | TGGGCTGGGGAGGGACTTTCAGT | 62 | 349 |
| CRYBB2–2 | CCTTCAGCATCCTTTGGGTTCTCT | GCAGTTCTAAAAGCTTCATCAGTC | 62 | 330 |
| CRYBB2–3 | GTAGCCAGGATTCTGCCATAGGAA | GTGCCCTCTGGAGCATTTCATAGT | 62 | 360 |
| CRYBB2–4 | GGCCCCCTCACCCATACTCA | CTTCCCTCCTGCCTCAACCTAATC | 62 | 230 |
| CRYBB2–5 | CTTACCCTTGGGAAGTGGCAATGG | TCAAAGACCCACAGCAGACAAGTT | 62 | 600 |
| CRYGC-1 | TGCATAAAATCCCCTTACCG | CCTCCCTGTAACCCACATTG | 62 | 514 |
| CRYGC-2 | TGGTTGGACAAATTCTGGAAG | CCCACCCCATTCACTTCTTA | 60 | 430 |
| CRYGD-1 | CAGCAGCCCTCCTGCTAT | GGGTCCTGACTTGAGGATGT | 60 | 550 |
| CRYGD-2 | GCTTTTCTTCTCTTTTTATTTCTGG | AAGAAAGACACAAGCAAATCAGT | 62 | 308 |
| CRYGS-1 | GAAACCATCAATAGCGTCTAAATG | TGAAAAGCGGGTAGGCTAAA | 60 | 229 |
| CRYGS-2 | AATTAAGCCACCCAGCTCCT | GGGAGTACACAGTCCCCAGA | 60 | 319 |
| CRYGS-3 | GACCTGCTGGTGATTTCCAT | CACTGTGGCGAGCACTGTAT | 60 | 491 |
| Cx46–1 | CGGTGTTCATGAGCATTTTC | CTCTTCAGCTGCTCCTCCTC | 60 | 450 |
| Cx46–2 | GAGGAGGAGCAGCTGAAGAG | AGCGGTGTGCGCATAGTAG | 60 | 450 |
| Cx46–3 | TCGGGTTCCCACCCTACTAT | TATCTGCTGGTGGGAAGTGC | 62 | 300 |
| Cx50–1 | CCGCGTTAGCAAAAACAGAT | CCTCCATGCGGACGTAGT | 62 | 420 |
| Cx50–2 | GCAGATCATCTTCGTCTCCA | GGCCACAGACAACATGAACA | 62 | 330 |
| Cx50–3 | CCACGGAGAAAACCATCTTC | GAGCGTAGGAAGGCAGTGTC | 62 | 350 |
| Cx50–4 | TCGAGGAGAAGATCAGCACA | GGCTGCTGGCTTTGCTTAG | 62 | 500 |