Figure 6. Effects of PKA activator mp-cAMP or PKA inhibitor H-89 on AQP5 protein localization. MDCK cells expressing AQP5 were exposed to: mp-cAMP (A); H-89 (B); H-89 first and then stimulated with mp-cAMP (C). D-G: Mouse corneal epithelial AQP5 regulation studies in organ culture. Mouse eyes were dissected out and cultured in MEM containing 2% FBS with mp-cAMP (D, E) or H-89 (F, G) for 30 min (D, F) or 6 h (E, G). MDCK cells expressing AQP5 (A-C) and cryosections of cultured eyes were immunostained for corneal AQP5 expression (D-G). Epi: epithelium; Str: stroma.