Figure 5. Immunolocalization of AQP5 protein in comparison with AQP1 or AQP0 in mouse lens. A: AQP1 expression in the WT lens anterior epithelial cells; a window of anterior epithelial cells is enlarged and shown below, in the same figure. B: AQP5 expression in the WT lens epithelial cells. C: (negative control), AQP5 knockout mouse lens section showing lack of immunoreactivity in the epithelial cells. D: WT lens outer cortex fiber cells with AQP5 expression. E: WT lens inner cortex fiber cells with AQP5 expression. F: AQP0 expression in the lens inner cortex fiber cells (very intense immunoreactivity compared to AQP5 expression shown in E). G: WT lens equatorial region showing AQP5 in the epithelial and fiber cells. H: The window shown in G is enlarged to provide a clear view of anti-AQP5 antibody binding to the narrow end of the fiber cells. I, J: AQP5 knockout mouse lens sections showing lack of immunoreactivity in the cells at the equatorial and anterior regions, respectively. A-J: FITC conjugated secondary antibody; green, antibody binding indicating AQP expression; blue, nuclear stain DAPI. White arrows – antibody binding. Yellow arrows – narrow side of the fiber cell; Red arrows – broader side of the fiber cell; A-C and G-J Sagittal sections; D-F: Cross sections.