Figure 3. Immunoblot analyses of corneal and lens membrane proteins to identify the expression of AQP5. A: From rabbit: Lanes; 1. total cornea, 2. total lens. B: From mouse: Lanes: 1. lacrimal gland (+ve control), 2. total cornea, 3. total lens, 4. lens epithelial cell membrane, 5. lens cortex fiber cell membrane, 6. lens nuclear fiber cell membrane. C: AQP5-KO mouse lens fiber cell membrane proteins. Lanes: 1. AQP0, 2. AQP5, expressions studied using anti-AQP0 and anti-AQP5 antibodies, respectively. D: Immunoblot of dephosphorylation studies in WT corneal membrane proteins using anti-AQP5 antibody. Lanes: 1. WT untreated proteins (arrow - ~28 kDa; arrowhead - ~34 kDa), 2. WT proteins treated with calf intestinal alkaline phosphatase; the 34 kDa band disappeared, presumably due to dephosphorylation.