Figure 1. Pretreatment with intravitreal bevacizumab (IVB) significantly reduced the numbers of vascular endothelial cells in neovascular membranes (NVMs) of the eyes with proliferative diabetic retinopathy (PDR). Hematoxylin and eosin stain (H&E; A, C, and E) and von Willebrand stain (B, D, and F) were applied to detect the vascular endothelial cells in the NVMs of PDR eyes (A-D) and epiretinal membranes of the eyes with proliferative vitreoretinopathy (PVR; E, F). The untreated group (A, B) shows a significantly more number of vascular endothelial cells when compared to the IVB pretreated group (C, D; p=0.003). The epiretinal membranes of PVR eyes were set as the control group. von Willebrand stain for vascular endothelial cells in the control group was negative (F). Figures were selected as representative data from three independent experiments. Scale bars: 200 μm.