Figure 4. Effect of ocular hypertension (45 mmHg for 7 h) and RGC loss over 28 days. DTMR-labeled retinal ganglion cell (RGC) density was examined at designated time points after IOP elevation. A: Representative 40× images from the central retina, showing a time-dependent loss of RGC. B: Quantitative comparison of RGC densities between a naïve rat (Time 0) and different days after ocular hypertension (45 mmHg for 7 h). Symbols represent mean±SEM (n=6). ^#: p<0.05 versus control using a one-way ANOVA and a Dunnett’s test. *: p<0.001 versus control using a one-way ANOVA and a Dunnett’s test.