Figure 3. Time-dependent morphological changes of the rat retina induced by ocular hypertension (IOP=45 mmHg). A: Representative images of retina cross-sections from animals with 5, 6, or 7 h of induced ocular hypertension. The sections were stained with hematoxylin and eosin and the thicknesses of various retinal layers and cell densities in the ganglion cell layer (GCL) were measured. Untreated eyes served as controls. Thicknesses of the overall retina [OLM-ILM] (B), inner retinal layer [IPL-ILM] (C), ONL (D), OPL (E), INL (F), and cell density in the GCL (G) were quantified and are shown. Bars represent mean±SEM *: p<0.001 versus control using a one-way ANOVA and a Dunnett’s test. GCL: ganglion cell layer; ILM: inner limiting membrane; INL: inner nuclear layer; IPL: inner plexiform layer; OLM: outer limiting membrane; ONL: outer nuclear layer; OPL: outer plexiform layer.