Figure 3. UVB-induced corneal damages, central corneal epithelial thickness, NF-κB and iNOS expression, and MDA accumulation among study groups. A-E: Hematoxylin-Eosin (HE) stain of corneas showed much thinner corneal epithelial layer with UVB exposure and the reverse effect with dietary zerumbone. U: The central corneal epithelial thickness was significantly reversed with dietary zerumbone, starting at 10 mg/kg. F-T: Immunohistochemical staining showed evident inhibition of NF-κB and iNOS expression with dietary zerumbone supplementation at 100 mg/kg and 10 mg/kg, respectively. NF-κB nuclear translocation in the UVB-exposed cornea in (F) was evidently reversed in the cornea with dietary zerumbone at 100 mg/kg in (I). MDA accumulation was reduced with dietary zerumbone supplementation at 100 mg/kg. All scale bars in E, J, O, and T are equal to 20μm.