Table 1 of Karolak, Mol Vis 2011; 17:827-843.

Table 1. Primer sequences and annealing temperature used to PCR amplifications of COL4A1 and COL4A2 fragments.



Name

Forward

Reverse		 Annealing
Temperature
(°C)		 Amplicon
Size
(bp)
COL4A1.1 CACCCTCCCCCTTTCTACTC GCCCAGAGAATGCACCTG 59 837
COL4A1.2 TTGGGCTGAGTAACACTTGG GCCTGGTTTGGCTTCATTTG 58 459
COL4A1.3–4 GGGCAACAGAATGAGACTCC TGTGAGCTGGGAGAGGAGAT 66 477
COL4A1.5–6 TGCTCTGTCTGCTTTGTGTG ACAAGCTGTGCTACTGGGTA 60 698
COL4A1.7–8 CCAACAAATGAAGGGTAGGG TGTGCCAAGTGTCTGAACG 58 578
COL4A1.9–10 CCTTTGCTTTGCCGTCTCTA TCATCATCCCTTTCCCACAG 60 691
COL4A1.11 GGAGATGGATTGGTATTGGT GACTAAGGGATGGATGAAAG 58 451
COL4A1.12 GGGACAAAGCTATTGCCTGA GACATTGATCCAAAGGTGGG 58 239
COL4A1.13 GCAGAGGCAAGGATGATTAG GGGGCTCGTATTTTATGGAC 58 393
COL4A1.14–15 CCCTGCCCTGCTTACATT GTCCCTACGAGCCTTTTCTG 60 505
COL4A1.16–17 TTAGTGGAGACGGGATTTCG AACTGCCTGCTTGTGTATGC 60 725
COL4A1.18 GATGGGACAAGTATCTGGGC CATCTCCTCTCCTTCCTCTC 60 459
COL4A1.19 GCTACCATTGCTGCTACTTCAC AATAGAAAGCGTGGGGAGAG 62 447
COL4A1.20 GTCACAACAGGCTTCAGGAG CCCAGGAGAGACATAAGGGT 60 486
COL4A1.21 CAGTGATGGTCTGGTTGGAT ATGCCAGGAGTCTCAGAGGT 60 532
COL4A1.22 TGGGTGGTGTGTGGTGATTA GAGAAGGGGCAAAACTCTGA 60 516
COL4A1.23 TTCCACCCATTAGCAGAGAG GCCAACACACCAAAGCAA 60 304
COL4A1.24 GTCCGTCTTGGGCATTTTAG ATTTGGGCTCTGTGGGTAAC 60 431
COL4A1.25 GTGCCCAAAGCCACACTATA TGTTCAGTTCCCCCAAATGC 60 718
COL4A1.26 CCTGGGAGGGTAGATGAAGT GAAAGGGAGGCACAAAAGG 62 488
COL4A1.27–28 AAGTGGAGAACACAGGCAGA TCTTCCCAACCAAACCCTAC 56 636
COL4A1.29 AGGTGCTGGAAGAGACAGCA GCTGAGGCTGAGAAACCATC 60 678
COL4A1.30 GCTTGAAAAGGGTTGAGCAG GGCCTCTAAGATTTGCATCG 64 315
COL4A1.31 CAGAGCCCCTACCGAGTATA CAGTGGGTGGGAGAAGAATC 61 483
COL4A1.32–33 CATTCAAGTTCCCAGTGTGG GCCTTCTGCTTGATGTTCCT 60 653
COL4A1.34 CTCATTTACCTGGGGTTGGA TATGGAGGACCCGATAACCC 60 411
COL4A1.35–36 TGTGCCTTTCCTGGGTTATC AATGTCATCCATCCCTGAGC 64 594
COL4A1.37 GGGGGATTCACGTTCTTGTA TCCCTGTGTGTTATGGCTCA 58 364
COL4A1.38–39 TGGCAGGTAGAAACCAGATG TGAAGATGGGAGACAGGACA 61 641
COL4A1.40 GACCTCAGGAAAACCAGGTG GTAGTTGCAGGGATGTGCAG 60 359
COL4A1.41 TGGTGGTTCTGAGCTGAAAG CATGTGTCTTGCAGGCATTG 60 447
COL4A1.42 TAAAGAGAAGGAGGGATCGG TCTTCACCAGAACCCACAAG 60 673
COL4A1.43 CCTGCCTCGATTTCTGTCTC TAGTGGGGATGTGGGAGTGT 60 435
COL4A1.44 CCACAAGGCACCATTTGTTC TACAAATTGGGCTGCCACAC 60 376
COL4A1.45 GGACCAAAAACAGTGCCCTA GAGCCTTGGGAAGTTTCTGA 60 790
COL4A1.46 CCAGAATGCAGTGGGAAGTT TTCCTGGGTTTTCTTCTGGA 60 590
COL4A1.47 ACAGCAAGAAACCAGGGAGA GGCTGCCTTTCAACAACATC 60 591
COL4A1.48 TGAAGGAGGTAGGCTGCTGT CGCAGTGTTTCACTCGCTAC 60 516
COL4A1.49 TGTTGTGAAAGACATTGCCC GCCCAGCCAACTGACTTTTA 60 650
COL4A1.50 AAAACCAACGGGGAGGTACT TAAGCAGCGAGATGCAGAGA 59 407
COL4A1.51 GGAAGCAGCCATTAGACGAT AAATCGTCTCGGTCATCTGC 60 573
COL4A1.52.1 TACCAGGTTGAGGCCTGATG ACCTCCTAGCACCCTTTGGT 65 530
COL4A1.52.2 GAAAACCAAAGGGTGCTAGG CCGAATGTGCTTACGTGTGA 65 793
COL4A1.52.3 CCTGGCTTGAAAAACAGCTC AATCACCCCCAGTCTGTGAC 60 429
COL4A2.1 TCGTGGGAAAGCTCAGATAC AGACAAAGCGAGTTTAGCGC 60 1454
COL4A2.2 GCTTCTGGAAGGGCCAAT GGGAAAGGGAGGAAGAGAGA 60 587
COL4A2.3 CCTCATCCTGCGCTAAACTC ACACTTTCCTGGCCTCTACG 60 625
COL4A2.4 ATTTCAGGGGTGGGAGAGAC CGGCCATCTAGGTTTGTGTG 60 467
COL4A2.5–6 TTCTTTCATCCCAACCCAGT TCCCCACGTGTTTTATGTCA 59 663
COL4A2.7 AGACAGAAGAAACCCCGACA TCTTGGGCGTCAACATACAG 60 515
COL4A2.8 TCAGAATAACCCCCATCAGC AACAGATCAGCCCTATCAGGAC 60 568
COL4A2.9–10 AGGTCCTGATAGGGCTGATCT TAACTGGCAGAGAGCTGGTG 59 551
COL4A2.11 GCATCAGAAACCTCCATGC ACATTGGCCTCCCTACAACA 59 556
COL4A2.12 TCCAATCTCAGCTCCCACTC TGTCCTCACCTCCACCTTCT 60 548
COL4A2.13 GGAAACAACCCCACAGAAAC GGAGGACCCGGTTATGTTTT 59 524
COL4A2.14 GTAAACATCTGCCTGGAACG CTATGGACAAGGGGATGAGA 58 469
COL4A2.15 TGTCACTGCCTGTCCTCAGA CCCCAGTGCTAGATGTTCGT 61 513
COL4A2.16 ATTATTTCCCATCCCCACCT GCAAAAATGAGAGCCAAGGT 59 473
COL4A2.17 CCCAGTGTCTTCAACAACCA TGTCAGAGGCCGTGTATTTG 59 505
COL4A2.18 AGCACAGTCTCCTGGCATTC CAGGCAACATGAAGGTCTCC 60 569
COL4A2.19 TTCGAGCTTTGGACTCACCT CTGTGAAGGTGTCCAAAGCA 60 521
COL4A2.20 ACCCATCGGAGTTATTGACG TACAGGGCTTCAGCTTCCAT 60 490
COL4A2.21 CCTGCATCTGTGGTTGTCTC AAGTTCGCCTCCTCATCAAC 59 609
COL4A2.22 CCTCTGAATGTGGTCCCAGT AAAGTCCGCCTTGGGGTAT 59 602
COL4A2.23–24 ATCGCAGAAAGTGCTCCTTG ATGAGCAGCCTGTCCTATGC 60 545
COL4A2.25 TGGCACTAGGTTCCTGTTCA ACAGGAGAGGCTGCATGTTT 59 553
COL4A2.26 AAACATGCAGCCTCTCCTGT TTCTGACAAGAGGGGTTTGG 60 492
COL4A2.27 CCAGAATGGTAGCCGGTTT GCAAGACCAGTTTGTGCTGA 60 318
COL4A2.28 TAAGCCTGGAGGTGCTGTTT CCGAAACACCTGTCTCCTTT 59 499
COL4A2.29 GCGAAGGTTGTAGGTTCCAA TGCCAAGACAAACAGTGAGC 60 708
COL4A2.30 GAATAGACAAGGGCAGGAAGG CAGAGGATGAGCCGATGTCT 60 581
COL4A2.31 CACAGCCTCAACCTCCAGAT CAGGCAGGAGCAGTTTGTCT 60 643
COL4A2.32 TGCTCCTCTGCCTTTGTCTT TGTTGAGGCAGGGATAAAGC 60 656
COL4A2.33 TGGTCTCTCTCCAAGGCTTC ACCGAGGTTACTCAGGCATC 59 442
COL4A2.34 ACAGCACGTAGGACAGCAAA ACATCTGCATGGTGTCCAAG 59 470
COL4A2.35 GCTAAGCAAACCGCCTATGA ACAGGACTTTCCACTGGGACT 60 416
COL4A2.36 GGGAGTCCACAATTCAGAGC GACCCTTCGCTGTTTCTGAG 59 629
COL4A2.37 CCCATGCTTCTCTCCAATTC ATGCCTCTCTCCATTCCTGA 60 446
COL4A2.38 CTGCTGCTGCTTTCTGTGTT CCTGTGCTGCTATGTTGGTG 60 626
COL4A2.39 GTGCTGTCCCACACATGAAA AGTCCATTCAACCCAGCAAC 61 510
COL4A2.40 ATGGGCCTCGATCCTCTTAT AAACCAGCTCTTTCCTGCAC 60 484
COL4A2.41 CCCACCATGAGATGTTCCTT ATGACACAGGAGGAGCCATC 60 427
COL4A2.42–43 AGTCATTCCATGCCACAGAC TAAGCTCTCCATTCCCCAAG 60 666
COL4A2.44–45 CCCGTTAGTGTCTGGCTCAT AGGTGTTCTGCTGGGCATAG 60 744
COL4A2.46 GAAACTGCCCTGCACTCCT TAGATGGACCCTTCCGTCAG 60 664
COL4A2.47 CACTCCCTGGTGATCCAACT CCAACTACCCTTGTGCAGTG 60 675
COL4A2.48.1 GGATGCCTCATGTCCGTATT TACATGGGTGTGTGCGAAGT 60 689
COL4A2.48.2 CATCCAGCAGCAGCACTTAG AGGTCTCCACTTCTGCCTGA 59 530
COL4A2.48.3 CCTGCTTTCTACGCCAATGT CTGGTTGGGGTGTTTTCTGT 60 573

In the table, Amplicon Size represents length of the PCR product in base pairs (bp) and Annealing Temperature represents the annealing temperature of the primers used for PCR amplifications.

Karolak, Mol Vis 2011; 17:827-843. http://www.molvis.org/molvis/v17/a94

©2011 Molecular Vision http://www.molvis.org/molvis/

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