Table 1 of Watson, Mol Vis 2011; 17:737-744. Table 1 of Watson, Mol Vis 2011; 17:737-744.
Table 1. Sequence and details of qRT–PCR primers.
| Gene | Primer | Sequence | Notes |
|---|---|---|---|
| Vsx1 primer pair 1 | forward | TATGCCCCGTTTTAGTCTGG | both primers within exon 5; 143 bp product |
| reverse | CCCAAAACAGACAACCCTTC | ||
| Vsx1 primer pair 2 | forward | GGTTTTCACTGCCCATCAAC | reverse primer spans exon 3/4 junction; 109 bp product |
| reverse | TTCCGGGAGCTCTGTTTTC | ||
| Vsx1 primer pair 3 | forward | TGGGGATGCATAAAAAGTCC | forward primer spans 4/5 junction; 146 bp product |
| reverse | GTTCTGGGTTGTTTCGTCTG | ||
| Gapdh | forward | TGTGTCCGTCGTGGATCTGA | 77 bp product [20] |
| reverse | CCTGCTTCACCACCTTCTTGA | ||
| Rho (rhodopsin) | forward | TTGCCACACTTGGAGGTGAA | forward primer spans exon 1/2 junction, 70 bp product [21] |
| reverse | ACCACCACGTAGCGCTCAAT | ||
| Acta2 (alpha 2-SMA) | forward | ACTGGGACGACATGGAAAAG | 240 bp product [22] |
| reverse | CATCTCCAGAGTCCAGCACA |