Figure 4. Vsx1 qRT-PCR expression is not detected in the cornea following alkali wounding. orneal wounding was performed through application of 0.2 M NaOH, which resulted in significant structural changes after 3 days between untreated (A) and treated (B) mouse eyes. Relative expression of Vsx1 in wounded versus unwounded eyes, normalized to Gapdh, from retinal and corneal samples is shown along with retinal Rho and Acta2 expression. y-axis shows relative expression between wounded and untreated eyes while the x-axis indicates primer set (Vsx1 sets 1–3, Acta2, and Rho) and tissue (retinal or corneal).