Table 1 of Hu, Mol Vis 2011; 17:715-722. Table 1 of Hu, Mol Vis 2011; 17:715-722.
Table 1. Primers and PCR conditions used to amplify genomic segments of GPR143 and FRMD7.
Primer name |
Forward primer(5’-3’) |
Reverse primer(5’-3’) |
Annealing temperature (°C) |
Product size (bp) |
|---|---|---|---|---|
| GPR143-1a | CTCCTCCGCCCGCCCAAGCATCAC | CCCAGGCAGCCGAGAAGGTC | 66 | 464 |
| GPR143-1b | CCGCGCCTAGGGACCTTCTGCT | AACCCGCGGGCCTCTCGTCCTCAC | 69 | 399 |
| GPR143-2 | CTTTCTTCCTTTTCCCTCCTTGTC | GTTTGCTGCTGCTGCGATTTG | 61 | 360 |
| GPR143-3 | CACGTGCGGCTTCCTGAC | TTGGCCTCTTATAAAAATGA | 59 | 385 |
| GPR143-4 | GGGCTTTCCTCTGTGTACATTTTC | CCCTGAGACAACGGCCTAACC | 63 | 334 |
| GPR143-5 | GCATTTCCCTTTTTGTTCTCATCC | AGGCCTGCACATTTTCATTTATTG | 61 | 406 |
| GPR143-6 | TTGCTTCCTGCCCCTCTGG | ACTTGCTCCCCTGTCCTCTGT | 63 | 400 |
| GPR143-7 | TGCACCTGGCCCTCTTAGTTTC | TCAGGAGGCCAAGACAGAGGAT | 63 | 441 |
| GPR143-8a | AAACCAACCCACCAACCAGTCAAC | GCATGCTCAGGGCTTCGTCA | 63 | 395 |
| GPR143-8b | CCAGCCCAGGGATTTCTCTT | ACCCCGCCATGCACAGGAC | 63 | 329 |
| GPR143-9 | AGCTGATGACAAACCTGCTAG | CCCTTTCTCCTATCCTAAAG | 61 | 330 |
| FRMD7-1 | CCTTGGGTGTGCATTACTTC | TTTGCTATTGTTGTCCCTTGAG | 57 | 459 |
| FRMD7-2 | AAACAACACAGAGACAGATAAGTGG | CAATCAGGGAATTGAACCCTAC | 57 | 385 |
| FRMD7-3 | AGGCAGTGGAGCAGTGATTC | GCAGCATGATTTCTTTCATCTC | 68 | 499 |
| FRMD7-4 | CTCGAAGGCAGAGAGGGTAG | CCCTTTGGATGATGAACACC | 69 | 519 |
| FRMD7-5 | GGCACCATTCCTTTCTTGAAT | CAGGCCATGCTGTTTCTCTC | 57 | 350 |
| FRMD7-6 | TTTGGACTGCATTGGCTACA | AGGATCTCAGCGTTTCATGG | 57 | 353 |
| FRMD7-7 | TCATGCACTTTCATCAGAAGC | TGATTGACCATTTCCCTTTC | 57 | 497 |
| FRMD7-8 | TGTGCAAGAGATGGGTCAAG | CTCTGGTTGATTTCTTCAAAGG | 57 | 368 |
| FRMD7-9 | GCTCTGTTTGTGAGCAGTGG | AGGGTGCAATCTTTGATGTG | 57 | 495 |
| FRMD7-10 | AGGTTGTTCTCTGCCTGGTC | GCACTGTCGTTCATGGTACTG | 57 | 398 |
| FRMD7-11 | TGTTTCTCTTGCTCGTGTTGA | TTTTTACACACTGGGATTCTGG | 57 | 282 |
| FRMD7-12a | CCCTAGAATAGAACATGGATCTTG | TGGGATCAGGGTTAGGATTG | 57 | 388 |
| FRMD7-12b | CCTTCTTTCACCAATGTGTCC | AATACCAACCTGCTGACCTG | 57 | 452 |
| FRMD7-12c | CTTTAACACTGAGCCCAATC | TGACTGAGAGCAGGACAAGG | 57 | 588 |
| FRMD7-12d | ACGGATGTGCCCTATATTCC | GCAACTCCTGCTCTGCAAAC | 57 | 472 |
| FRMD7-12e | AGCCCAAGGAATATCAGAATG | GCAGTTGGTGTGTTGAAATAAGC | 57 | 500 |
| FRMD7-12f | GCTCTCAGTCATAAAGCAGACC | CCTTCAGAGGTAATGGAAGAGTG | 57 | 500 |