Figure 4. Sequencing of GPR143. A: Sequence in the proband (V4) showing a novel splicing mutation c.658+1G>T. B: Sequence in the proband's mother (IV9) revealing a heterozygous mutation. C: The sequencing result of the fetus (V5) hemizygous for the mutant allele. D: Sequence in an unaffected male member (III6) hemizygous for the wild type allele.