Table 1 of Kumar, Mol Vis 2011; 17:693-707. Table 1 of Kumar, Mol Vis 2011; 17:693-707.
Table 1. Oligonucleotides used as primers for PCR amplification of CRYAB, CRYGC, CRYGD, and GJA8 and their annealing temperatures.
| Gene (exon) | Forward primer (5′→3′) | Reverse primer (5′→3′) | Temperature (°C) |
|---|---|---|---|
| CRYAB (1) | CCTGACATCACCATTCCAGA | GGCAGGGTAGGAAAGGAAA | 51 |
| CRYAB (2) | TGCAGAATAAGACAGCACCTG | CCAGCCTCCAAAGCTGATAG | 54 |
| CRYAB (3) | TGTTGTCATGGCATTTGGTC | TCATTCACTGGTGGGGAAA | 57 |
| CRYGC (1,2) | CAGCCATCCTGCTATATAG | GGCATGATGGAAATCTAG | 50 |
| CRYGC (3) | GTTGGACAAATTCTGGAA | GCACAATGAAAGAATGAC | 45 |
| CRYGD (1) | AGAACACGAAAATGCCCTTG | GTCTCACAGGCCTGCTCCT | 55 |
| CRYGD (2) | GAGCTTCCTCCATCGC | CCTGGGTCCTGACTTGA | 48 |
| CRYGD (3) | GCTGGACTGCCTAACAATGC | CACATCTTGGTTGCCATTTG | 55 |
| GJA8 (1) | TATGGGCGACTGGAGTTTCCT | CTCCATGCGGACGTAGTGCAC | 65 |
| GJA8 (2) | CTCTGGGTGCTGCAGATCATC | CACAGAGGCCACAGACAACAT | 55 |
| GJA8 (3) | CACTACTTCCTGTACGGGTTC | CTCTTGGTAGCCCCGGGACAA | 60 |
| GJA8 (4) | GTCTCCTCCATCCAGAAAGCC | TCATACGGTTAGATCGTCTGA | 58 |