Figure 5. Expression of corneal differentiation marker in mouse corneas after limbus to limbus epithelial scraping and treatment with conditioned media. Corneas treated with skin conditioned media showed no K12 (A, B) with abundant expression of K8 (C, D). Eyes treated with limbal fibroblast conditioned media demonstrated K12 staining (E, F) and reduced K8 positive cells (G, H).