Figure 3. Expression of keratin 8 (K8) and keratin 12 (K12) in normal and limbal deficient mouse corneas. Cross sections of normal (A-D) and limbal stem cell deficient corneas after limbus to limbus epithelial debridement (E-H). Normal corneal epithelium expressed K12 (A, B) but no K8 staining (C). Staining for K8 is evident only in the conjunctiva and limbal area (D). By contrast, limbal deficient corneas demonstrated no K12 (E, F) but abundant K8 (G, H) staining. Nuclei are stained with DAPI in blue.