Figure 2. Expression of keratin 8 (K8) and keratin 12 (K12) in normal and limbal deficient mouse corneas. Normal (A-C) and limbal stem cell deficient corneas after limbus to limbus epithelial debridement (D-F). Wholemount staining shows the absence of goblet cell marker K8 (green, A), and the presence of K12 (red, B) in a normal cornea while in limbal deficient corneas there was the presence of K8 (green, D) and absence of K12 (red, E). An overlay of the K8 and K12 staining is shown for normal (C) and limbal deficient (F) corneas. High magnification of the double staining along with DAPI staining (blue) is shown for normal (G) and limbal deficient (H) corneas.