Table 1 of Wang, Mol Vis 2011; 17:647-657. Table 1 of Wang, Mol Vis 2011; 17:647-657.
Table 1. Sequences, annealing temperatures (Tm), and predicted product sizes of the primers used.
| Gene | Upstream primer | Downstream primer | Tm (°C) | Size (bp) | NCBI accession number (rat and human) |
|---|---|---|---|---|---|
| BMP-2 | GCGGAAACGCCTTAAGTCCA | GTGGAGTTCAGATGATCAGC | 55 | 156 | NM_017178.1 , NM_001200.2 |
| BMP-3 | TTGGCTGGAGCGAATGGATTA | GCTCAGGAATCCCAGAGACGAC | 56 | 159 | NM_017105.1 , NM_001201.2 |
| BMP-4 | TTTGTTCAAGATTGGCTCCCAAG | AAACGACCATCAGCATTCGGTTA | 55 | 101 | NM_012827.2 , BC020546.2 |
| BMP-5 | TCCACAGAACAATTTGGGCTTACA | ACCATGAACGGCTGCTTTGAC | 55 | 120 | NM_001108168.1 , NM_021073.2 |
| BMP-6 | CGCCTTCCTCAACGACCGCGG | GGAATCTGGGATAAGTTGAA | 55 | 120 | NM_013107.1 , NM_001718.4 |
| BMP-7 | TCCGGTTTGATCTTTCCAAGA | CCCGGATGTAGTCCTTATAGATCCT | 55 | 81 | NM_001191856.1 , NM_001719.2 |
| BMPR-IA | GCCGTTTTGAAGCTGATGTCA | TCTTTGCGAGCGTCTTCTTGA | 55 | 88 | S75359.1 , NM_004329.2 |
| BMPR-IB | TGGCTGACATGTACAGCTTTGG | GGCACTCGTCACTGCTCCAT | 56 | 198 | NM_001024259.1 , D89675.1 |
| BMPR-ll | ACTGCAGATGGACGCATGG | AATCTCGATGGGAAATTGCAG | 55 | 199 | NM_080407.1 , NM_001204.6 |
| Smad1 | CTCCAATGTTAACCGGAACTCCAC | CTCTGCACGAAGATGCTGCTG | 56 | 129 | NM_013130.2 , U59423.1 |
| Collagen I | TGCTGGCAAGAATGGCGATC | CTGTCTCAGCCTTGTCACCAC | 55 | 122 | NM_000088.3 , AF169346.1 |
| aggrecan | GAAGTGATGCATGGCATTGAGG | ATGATGGCGCTGTTCTGAAGG | 55 | 146 | NM_022190.1 , BC036445.1 |
| β-actin | GGCACCACACTTTCTACAATG | GGGGTGTTGAAGGTCTCAAAC | 55 | 133 | NM_031144.2 , NM_001101.3 |