Figure 6. Potential effect of BMP-2 on the signal pathway in vitro. A: The mRNA expression in BMPRs, smad1, and BMP-2 after 24 h of stimulation with 100 ng/ml BMP-2. B: Immunofluorescent staining of Smad1/5/8 phosphorylation located in the cells treated by 100 ng/ml BMP-2 for 1 h.The second antibody is Cy3-labeled goat anti-rabbit (Boster) and nuclear was stained with DAPI. C: The protein level of phospho-Smad1/5/8 in the treated cells detected by western blot.