Figure 6. Involvement of FAK phosphorylation and integrin signaling with adhesion of HCECs to F. solani spores. After pretreatment with/without genistein, HCECs were exposed to F. solani spore suspensions. Western blot assay showed that 5 h after incubation, p-FAK production was significantly increased (A). The graph (B) compares scanning signal intensity of p-FAK expression by ImageJ software. The expression of p-FAK greatly increased (p<0.001) in all treated groups (7 and 8 h). There were significant differences for the phosphorylation levels of FAK in all treated groups. *p<0.01, **p<0.001. The β1 integrin and p-PAX from cells incubated with F. solani spores were also analyzed by western blot (C). The graph (D) compared scanning signal intensity of β1 integrin expression by ImageJ software and indicated the significant overexpression (p<0.001) of β1 integrin protein in all treated groups (6, 7, and 8 h). The data showed no significant differences (p<0.05) between the genistein treated and non-treated groups. The expression of p-PAX was significantly increased (p<0.001) in all treated groups and when the cells were pretreated with genistein, the expression of p-PAX was significantly lower (p<0.001) than in the no-genistein treated group (E). GAPDH was used as a loading control.