Figure 2. Subcellular localization of full-length FRMD7 versus various domains of FRMD7. HEK293T cells transiently transfected with EGFP-fusion proteins of FRMD7 (green) were stained with DAPI (blue) and imaged. Full-length FRMD7 was diffusely localized to the cytoplasm, while the NH₂-terminal FERM domain (aa 1–279), and the FERM domain plus the FA domain, primarily localized to the nucleus (arrows). Constructs containing the COOH-terminal region of FRMD7 (e.g., △FERM and △FERM+FA) exhibited an expression pattern similar to that of full-length FRMD7, however, the expression level was decreased and some aggregates of these truncated proteins were detected in the cytoplasm. The results obtained from Neuro-2a cells (not shown) was the same as HEK293T cells. FERM: four-point-one, ezrin, radixin, moesin; FERM+FA: FERM domain and FERM Adjacent domain; △FERM: truncated NH₂-terminal FERM domain; △FERM+FA; truncated NH₂-terminal FERM domain and FERM Adjacent domain. Scale bars: 20 μm.