Figure 2. Radial sections demonstrating second-harmonic generation and autofluorescence of the TM region of a human eye. Multiple tiled scans were performed and projected into three dimensions. Shown here are snapshots every 15° of rotation about the y-axis. As the tissue rotates about the y-axis, the scleral spur, iris root (white arrow) and attached TM tissue (dotted lines) can be seen with considerable detail. Schlemm’s canal (red circle) can be seen clearly adjacent to the TM. White bar=200 µm.