Figure 1. A histological section of the same human trabecular meshwork (TM) region imaged by 2PM. The paraffin section was stained with hematoxylin and eosin and placed alongside a schematic outline. The TM region is clearly visible in the lower right-hand edge of each section (toward the anterior chamber). The TM region shows darkly-stained endothelial cells populating a pink-stained fibrous tissue region of the eye. Schlemm’s canal is visible proximal to the TM region (outlined in red in schematic). The scleral spur and iris remnant are also labeled in the schematic, as are multiple opens spaces formed during processing the tissue for histology (artifacts). Black bar=100 µm.