Figure 1. Angiogenin is expressed in the
human retina and retinal pigment epithelium (RPE)-choroid. A:
Reverse-transcription (RT)-PCR results. Angiogenin cDNA was amplified
from both human retina (Ret) and choroid (Ch) tissues. Experiments were
performed with (+) and without (−) reverse transcriptase enzyme, the
latter serving as controls. B: western blot results show that
the angiogenin protein is detectable only in the retinal pigment
epithelium-choroid samples (left panel). Recombinant human angiogenin
protein (rAng) was detected at the correct molecular weight
(14 kDa) with the anti-angiogenin antibody as a positive control.
The stripped blot, probed with anti-actin antibody (right panel) shows
even loading between retinal and choroidal samples.