Figure 1. Angiogenin is expressed in the human retina and retinal pigment epithelium (RPE)-choroid. A: Reverse-transcription (RT)-PCR results. Angiogenin cDNA was amplified from both human retina (Ret) and choroid (Ch) tissues. Experiments were performed with (+) and without (−) reverse transcriptase enzyme, the latter serving as controls. B: western blot results show that the angiogenin protein is detectable only in the retinal pigment epithelium-choroid samples (left panel). Recombinant human angiogenin protein (rAng) was detected at the correct molecular weight (14 kDa) with the anti-angiogenin antibody as a positive control. The stripped blot, probed with anti-actin antibody (right panel) shows even loading between retinal and choroidal samples.