Table 2 of Vaajasaari, Mol Vis 2011; 17:558-575.

Table 2. Reverse-transciptase (RT)–PCR Primer sequences.

  Primer sequences (5′ > 3′)  
Gene Forward Reverse Tann
GAPDH GTTCGACAGTCAGCCGCATC GGAATTTGCCATGGGTGGA 55
PAX6 AACGACACAGCCCTCACAAACA CGGGAACTTGAACTGGAACTGAC 60
RAX CTGAAAGCCAAGGAGCACATC CTCCTGGGAATGGCCAAGTTT 55
MITF AAGTCCTGAGCTTGCCATGT GGCAGACCT TGGTTTCCAA 52
RPE65 TCCCCAATACAACTGCCACT CACCACCACACTCAGAACTA 52
bestrophin GAATTTGCAGGTGTCCCTGT ATCCTCCTCGTCCTCCTG AT 55
OTX2v1 GGGCCCTGGGCTTCTTGTCC ATTGGCCACTTGTTCCACTC 52
PMEL GTGGTCAGCACCCAGCTTAT GAGGAGGGGGCTATTCTCAC 52
PEDF AGCTCGCCAGGTCCACAAAG TGGGCAATCTTGCAGCTGAG 60
tyrosinase TGCCAACGATCCTATCTTCC GACACAGCAAGCTCACAAGC 52
SOX10 AGCCCAGGTGAAGACAGAGA AGGAGAAGGCCGAGTAGAGG 55
alphafetoprotein GCTGGATTGTCTGCAGGATGGGGAA TCCCCTGAAGAAAATTGGTTAAAAT 55
alpha carciac actin GGAGTTATGGTGGGTATGGGTC AGTGGTGACAAAGGAGTAGCCA 55
nanog TGAAATGTCTTCTGCTGAGAT GTTCAGGATGTTGGAGAGTTC 55
OCT 3/4 CGTGAAGCTGGAGAAGGAGAAGCTG AAGGGCCGCAGCTTACACATGTTC 62

Abbreviations: Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), paired box gene 6 (PAX6), retina and anterior neural fold homeobox (RAX), microphthalmia-associated transcription factor (MITF), anti-retinal pigment epithelium-specific 65 kDa protein (RPE65), orthodenticle homeobox 2 variant 1 (OTX2v1), premelanosome protein (PMEL), pigment epithelium-derived factor (PEDF), sex determining region Y-box 10 (SOX10).

Vaajasaari, Mol Vis 2011; 17:558-575. http://www.molvis.org/molvis/v17/a64

©2011 Molecular Vision http://www.molvis.org/molvis/

ISSN 1090-0535