Table 1 of Vaajasaari, Mol Vis 2011; 17:558-575. Table 1 of Vaajasaari, Mol Vis 2011; 17:558-575.
Table 1. Summary of published retinal pigment epithelium (RPE) differentiation methods.
Reference |
Number of cell lines |
Feeder cells/ matrix for undifferentiated cells |
Serum/serum replacement for a) undifferentiated cells b) differentiation |
Differentiation matrix |
Serum free1 |
Xeno-free2 |
|---|---|---|---|---|---|---|
| [1] | hESC (11) | MEF | a) KO-SR, plasmanate | MEF, Gelatin; Feeder-free; EB | - | - |
| b) KO-SR, (plasmanate, FBS) | ||||||
| [17] | hESC (18) | MEF | a) KO-SR, plasmanate | MEF, Gelatin | - | - |
| b) KO-SR, plasmanate, FBS | ||||||
| [22] | hESC (2) | MEF | a) KO-SR | SFEB, Poly-D-Lysine-Laminin-Fibronectin | + | - |
| b) KO-SR | ||||||
| [50] | hESC (1) | MEF | a) KO-SR, FBS | Mouse PA6 cells, Human Bruch’s membrane, Matrigel™ | - | - |
| b) KO-SR | ||||||
| [37] | hESC (2) | MEF | a) KO-SR | MEF, Matrigel™ | + | - |
| b) KO-SR | ||||||
| [51] | hESC (1) | MEF | a) KO-SR | MEF, Matrigel™ | + | - |
| b) KO-SR | ||||||
| [52] | hiPSC (2) | mouse and human cells | a) FBS, KO-SR | SFEB, Poly-D-Lysine-Laminin-Fibronectin | - | - |
| b) KO-SR | ||||||
| [21] | hESC (1) hiPSC (4) | MEF, SNL | a) KO-SR | SFEB, Poly-D-Lysine-Laminin-Fibronectin | + | - |
| b) KO-SR | ||||||
| [32] | hESC (1) hiPSC (4) | hFF, MEF, MatrigelTM | a) KO-SR | Gelatin | - | - |
| b) KO-SR, FBS | ||||||
| [16] | hESC (4) | hFF | a) KO-SR | Floating clusters, Laminin | + | - |
| b) KO-SR | ||||||
| [34] | hESC (1) hiPSC (4) | MEF | a) KO-SR | Free-floating aggregates, Laminin | + | - |
| b) KO-SR, NIM, RDM | ||||||
| [33] | hiPSC(1) | MEF | a) KO-SR | MEF, Gelatin | - | - |
| b) FBS | ||||||
| [53] | hESC (2) | MatrigelTM | a) MEF-CM | Collagen-Laminin | - | - |
| b) Serum-free + FBS |