Table 1 of Vaajasaari, Mol Vis 2011; 17:558-575.


Table 1. Summary of published retinal pigment epithelium (RPE) differentiation methods.




Reference

Number
of cell
lines
Feeder cells/
matrix for
undifferentiated
cells

Serum/serum replacement
for
a) undifferentiated cells
b) differentiation



Differentiation matrix

Serum
free1


Xeno-free2
[1] hESC (11) MEF a) KO-SR, plasmanate MEF, Gelatin; Feeder-free; EB - -
      b) KO-SR, (plasmanate, FBS)      
[17] hESC (18) MEF a) KO-SR, plasmanate MEF, Gelatin - -
      b) KO-SR, plasmanate, FBS      
[22] hESC (2) MEF a) KO-SR SFEB, Poly-D-Lysine-Laminin-Fibronectin + -
      b) KO-SR      
[50] hESC (1) MEF a) KO-SR, FBS Mouse PA6 cells, Human Bruch’s membrane, Matrigel™ - -
      b) KO-SR      
[37] hESC (2) MEF a) KO-SR MEF, Matrigel™ + -
      b) KO-SR      
[51] hESC (1) MEF a) KO-SR MEF, Matrigel™ + -
      b) KO-SR      
[52] hiPSC (2) mouse and human cells a) FBS, KO-SR SFEB, Poly-D-Lysine-Laminin-Fibronectin - -
      b) KO-SR      
[21] hESC (1) hiPSC (4) MEF, SNL a) KO-SR SFEB, Poly-D-Lysine-Laminin-Fibronectin + -
      b) KO-SR      
[32] hESC (1) hiPSC (4) hFF, MEF, MatrigelTM a) KO-SR Gelatin - -
      b) KO-SR, FBS      
[16] hESC (4) hFF a) KO-SR Floating clusters, Laminin + -
      b) KO-SR      
[34] hESC (1) hiPSC (4) MEF a) KO-SR Free-floating aggregates, Laminin + -
      b) KO-SR, NIM, RDM      
[33] hiPSC(1) MEF a) KO-SR MEF, Gelatin - -
      b) FBS      
[53] hESC (2) MatrigelTM a) MEF-CM Collagen-Laminin - -
      b) Serum-free + FBS