Figure 7 of Vaajasaari, Mol Vis 2011; 17:558-575.


Figure 7. Differentiation of human pluripotent stem cells toward retinal pigment epithelium (RPE) cells under defined culture conditions, RPEregES. All represented images are from human embryonic stem cell (hESC)-RPE Regea 08/023. A: Reverse transcription (RT)–PCR analysis of typical genes for retinal/ RPE development expressed by undifferentiated hESC (Regea 08/023), human foreskin fibroblast (hFF) feeder cells, and putative hESC-RPE on D7 and D44. Expression of B: Microphthalmia-associated transcription factor (MITF), B: Cellular retinaldehyde-binding protein (CRALBP), and E,G: RPE65 on D83. F: For cell morphology, F-actins were stained using phalloidin. H: Proliferative activity was studied by Ki67 staining together with tight junction protein anti-zonula occludens (ZO)-1 in hESC-RPE. I: Vertical confocal sections showing apical localization of Na+/K+ATPase (green) and basolateral localization of Bestrophin (red). Nuclei stained with 4',6-diamidino-2-phenylindole (DAPI). Images B-D were taken with an Olympus BX60 microscope (Olympus, Tokyo, Japan) using a 60× oil immersion objective, scale bar 20 μm. Images E-I were taken with an LSM 700 confocal microscope (Carl Zeiss) using a 63× oil immersion objective, scale bar 20 μm.