Figure 6 of Vaajasaari, Mol Vis 2011; 17:558-575.


Figure 6. Phagocytosis of photoreceptor outer segments (POS) and cell membrane polarization of human embryonic stem cell (hESC; Regea 08/023) and human induced pluripotent stem cell (hiPSC; FiPS 5–7)-derived retinal pigment epithelium (RPE) cells. A: Putative hESC-RPE and B: hiPSC-RPE internalize POS (green, arrowheads) for cell morphology; F-actins were stained using phalloidin (red). Vertical confocal sections showing apical localization of Na+/K+ATPase (green) and basolateral localization of Bestrophin (red) in C: hESC-RPE and D: hiPSC-RPE. Images were taken with an LSM 700 confocal microscope (Carl Zeiss) using a 63× oil immersion objective, scale bar 20 μm.