Figure 6. Phagocytosis of photoreceptor
outer segments (POS) and cell membrane polarization of human embryonic
stem cell (hESC; Regea 08/023) and human induced pluripotent stem cell
(hiPSC; FiPS 5–7)-derived retinal pigment epithelium (RPE) cells. A:
Putative
hESC-RPE and B: hiPSC-RPE internalize POS (green,
arrowheads) for cell morphology; F-actins were stained using phalloidin
(red). Vertical confocal sections showing apical localization of Na+/K+ATPase
(green)
and basolateral localization of Bestrophin (red) in C:
hESC-RPE and D: hiPSC-RPE. Images were taken with an LSM 700
confocal microscope (Carl Zeiss) using a 63× oil immersion objective,
scale bar 20 μm.