Figure 3 of Vaajasaari, Mol Vis 2011; 17:558-575.


Figure 3. Immunofluorescence staining of human embryonic stem cell (hESC; Regea 08/023)- and human induced pluripotent stem cell (hiPSC; FiPS 5–7)-derived retinal pigment epithelium cells revealing maturation stage after 83 days of differentiation. Cellular retinaldehyde-binding protein (CRALBP) and microphthalmia-associated transcription factor (MITF) localization in A-C: manually selected hESC-RPE cells and D-F: hiPSC-retinal pigment epithelium (RPE) cells. G, I: RPE65 expression in hESC-RPE and J, L: hiPSC-RPE. H, K: For cell morphology, F-actins were stained using phalloidin. Tight junction protein anti-zonula occludens (ZO)-1 and proliferation marker Ki67 localization in M: hESC-RPE cells and N: hiPSC-RPE cells. Nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI). Images A-F were taken with an Olympus BX60 microscope (Olympus, Tokyo, Japan) using a 60× oil immersion objective, scale bar 20 μm. Images G-N were taken with an LSM 700 confocal microscope (Carl Zeiss) using a 63× oil immersion objective, scale bar 20 μm.