Table 2 of Zhang, Mol Vis 2011; 17:548-557.

Table 2. Primers sequences and the physical position used in real-time quantitative PCR and the results of MLPA and real-time PCR.

      Family 85 Family 86
Gene/SNP Physical position Primer sequences (5′-3′) MLPA RQ result* MLPA RQ result*
rs11031332 31235538 Fwd: CATTAGATTCATGTGGCTTGTTG  

1.17
    Rev: AATCCGATTCCTCGATTTGT        
rs1929020 31237204 Fwd: TGACTGGTGCTACTGCCAAC       0.49
    Rev: CTCCCGAATAGCGTGGATTA        
DCDC1 31240747–31347897   -   +  
ELP4 31487873–31761905   -   +  
rs35674517 31763272–31763273 Fwd: TTCTTCCCCATTTTCTTTTCAA   1.18    
    Rev: TTCAGAATGTTCATAATGCTTTCAA        
rs608293 31764856 Fwd: GAATATTTTTCCCCCAAAGC   0.44   1.07
    Rev: TGAGAGGCCCAGAGTAAAAAGA        
PAX6 31762916–31796085   +   -  
DKFZp686K1684 31794690–31865163   +   -  
RCN1 32074656–32083376   +   -  
SHGC-143238 32102437–32102880 Fwd: TCCTCCCCTTGAGTCACTTACAT   0.5    
    Rev: TTACTTGCCAAAACACACTCCCT        
rs7104670 32155485 Fwd: GTTACCACATTCGCAAAGCA   0.46    
    Rev: CAGACAAACACATTGACAGTCCT        
rs12577592 32165037 Fwd: CCTTGCTCCTTTCAGTCCAG   0.35    
    Rev: ACCCTGAGTGTGTGTCATGC        
rs4244480 32172819 Fwd: ACTTATCCCGCCCTTGTGTT   0.41    
    Rev: CACCAGGTTGTTTTGGGACT        
rs72320219 32173790–32173791 Fwd: AGGAAAATCGCTTGAACCTG   1.08    
    Rev: GGGGTGGTTGTGAGGACTAA        
GAPDH 6516714–6517797 Fwd: ACCCAGAAGACTGTGGATGG        
    Rev: TTCTAGACGGCAGGTCAGGT        

Abbreviations: MLPA, Multiplex ligation-dependent probe amplification; RQ, relative quantity; +, deletion ; -, no deletion; *mean value of three times; Fwd, forward; Rev, reverse.

Zhang, Mol Vis 2011; 17:548-557. http://www.molvis.org/molvis/v17/a63

©2011 Molecular Vision http://www.molvis.org/molvis/

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