Table 1 of Zhang, Mol Vis 2011; 17:548-557. Table 1 of Zhang, Mol Vis 2011; 17:548-557.
Table 1. PAX6 PCR primers used in this study
Primer |
Forward (5'-3') |
Reverse (5'-3') |
Tm (°C) |
Product size (bp) |
|---|---|---|---|---|
| exon1 | AGGGAACCGTGGCTCGGC | GGGTGAGGGAAGTGGCTGC | 62 | 207 |
| exon2 | TTATCTCTCACTCTCCAGCC | GGAGACCTGTCTGAATATTGC | 54 | 307 |
| exon3 | TCAGAGAGCCCATGGACGTAT | CTGTTTGTGGGTTTTGAGCC | 58 | 193 |
| exon4 | AGTTCAGGCCTACCTGATGC | GTCGCGAGTCCCTGTGTC | 58 | 201 |
| exon5 | CTCCCTCATCTTCCTCTTCC | GGGGTCCATAATTAGCATCG | 58 | 327 |
| exon6-7 | GGGCTACAAATGTAATTTTAAGAAA | AGAGAGGGTGGGAGGAGGTA | 56 | 509 |
| exon8 | GAGCTGAGATGGGTGACTG | GAGAGTAGGGGACAGGCAAA | 58 | 300 |
| exon9 | AGACTACACCAGGCCCCTTT | TGAAGATGTGGCATTTACTTTGA | 58 | 291 |
| exon10 | GGAACCAGTTTGATGCACAG | ACTCTGTACAAGCACCTCTGTCTC | 58 | 243 |
| exon11 | GGGCTCGACGTAGACACAGT | GGAAACTGAGGGCAAGAGAA | 56 | 300 |
| exon12 | CGGGCTCTGACTCTCACTCT | GCCACTCCTCACTTCTCTGG | 60 | 220 |
| exon13 | GCTGTGGCTGTGTGATGTGT | AGGAGATTCTGTTTGGGTA | 52 | 281 |
| exon14 | TCCATGTCTGTTTCTCAAAGG | TCAACTGTTGTGTCCCCATAG | 56 | 219 |