Figure 5. Analysis of deletions in the PAX6 gene region by real-time quantitative PCR. A: Fluorescence amplification plots of the real-time quantitative PCR for exon 8 of the PAX6 gene, detected deletion by MLPA. B: Fluorescence amplification plots of the real-time PCR for rs7104670. Arrows in A and B indicated triplicate signals obtained for a control subject (Normal) and the patient III-1 of family 85 (Patient). C: Histogram indicated the relative quantity (RQ) between the exon8 of PAX6 or rs7104670 and GAPDH values. Bars represent mean values±standard deviations for the triplicate values.