Figure 6. Effects of EGCG on IL-1β- and
hyperosmolarity-induced HCEpiC NFκB and AP-1 activity. Cells were
transfected with an AP-1 or NFκB reporter gene (expressing firefly) and
a constitutively expressing Renilla construct and then placed
in glucocorticoid-free medium for 24 h. Thirty h after transfection,
cells were treated with IL-β (panels A and B) or 440
mOsM media (panels C and D) in the presence or absence
of EGCG. Cells were assayed for firefly luciferase followed by Renilla
luciferase luminescence 48 h after transfection. Data are expressed as
normalized firefly: Renilla luciferase ratio. A: Effect
of EGCG on IL-1β-induced AP-1 transcriptional activity; B:
effect of EGCG on IL-1β-induced NFκB transcriptional activity. C:
Effect
of EGCG on 440 mOsm hyperosmolar-induced AP-1 transcriptional
activity; B: effect of EGCG on 440 mOsm hyperosmolar-induced
NFκB transcriptional activity. Data are mean±SEM, n=6. For A
and B, *versus IL-1β; for C and D, *versus 440
mOsm media; p<0.05.