Figure 6 of Cavet, Mol Vis 2011; 17:533-542.


Figure 6. Effects of EGCG on IL-1β- and hyperosmolarity-induced HCEpiC NFκB and AP-1 activity. Cells were transfected with an AP-1 or NFκB reporter gene (expressing firefly) and a constitutively expressing Renilla construct and then placed in glucocorticoid-free medium for 24 h. Thirty h after transfection, cells were treated with IL-β (panels A and B) or 440 mOsM media (panels C and D) in the presence or absence of EGCG. Cells were assayed for firefly luciferase followed by Renilla luciferase luminescence 48 h after transfection. Data are expressed as normalized firefly: Renilla luciferase ratio. A: Effect of EGCG on IL-1β-induced AP-1 transcriptional activity; B: effect of EGCG on IL-1β-induced NFκB transcriptional activity. C: Effect of EGCG on 440 mOsm hyperosmolar-induced AP-1 transcriptional activity; B: effect of EGCG on 440 mOsm hyperosmolar-induced NFκB transcriptional activity. Data are mean±SEM, n=6. For A and B, *versus IL-1β; for C and D, *versus 440 mOsm media; p<0.05.