Figure 5. Effects of EGCG on hyperosmolar-induced phosphorylated p38 and JNK levels in HCEpiC. Cells were cultured in complete (HCGS-containing) medium, followed by basal medium for 18 h. Cells were pre-treated with EGCG for 2 h. Cells were then treated with 440 mOsm hyperosmotic media + EGCG for 30 min. Phosphorylated p38 and JNK levels were determined by cell-based ELISA. A: effect of EGCG on hyperosmolar-induced phosphorylated p38; B: effect of EGCG on hyperosmolar-induced phosphorylated JNK; n=6, *versus 440 mOsm media; p<0.05.