Figure 2. EGCG inhibits hyperosmolar-induced cytokine release in HCEpiC. Cells were cultured in complete (HCGS-containing) medium, followed by glucocorticoid-free medium for 18 h. Cells were then treated with 440 mOsm hyperosmotic basal media in the presence of EGCG for 24 h. IL-6 (A) and MCP-1 (B) release into the media was analyzed by Luminex. White bar represents control (317 mOsm); black bar represents hyperosmolarity (440 mOsm); gray bar represents EGCG alone; black circles + line represents EGCG. Lines are the linear interpolation between data points. Data are presented as mean±SEM, n=3. *versus 440 mOsm hyperosmotic media; p<0.05.