Figure 5. Effect of triptolide on the poly(I:C)-induced activation of NF-κB in human corneal fibroblasts. Cells were incubated first for 1 h in the absence or presence of triptolide (3 nM) and then for 1 h in the additional absence or presence of poly(I:C) at 1 μg/ml. The cells were then subjected to immunofluorescence analysis with antibodies to the p65 subunit of NF-κB (green fluorescence), and nuclei were stained with TOTO-3 (blue fluorescence). Scale bar, 20 µm. Data are representative of three independent experiments.